De Novo Genome Assembly and Comparative Genomics of the Barley Leaf Rust Pathogen Puccinia hordei Identifies Candidates for Three Avirulence Genes.
Identifieur interne : 000274 ( Main/Exploration ); précédent : 000273; suivant : 000275De Novo Genome Assembly and Comparative Genomics of the Barley Leaf Rust Pathogen Puccinia hordei Identifies Candidates for Three Avirulence Genes.
Auteurs : Jiapeng Chen [Australie] ; Jingqin Wu [Australie] ; Peng Zhang [Australie] ; Chongmei Dong [Australie] ; Narayana M. Upadhyaya ; Qian Zhou [République populaire de Chine] ; Peter Dodds ; Robert F. Park [Australie]Source :
- G3 (Bethesda, Md.) [ 2160-1836 ] ; 2019.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Annotation de séquence moléculaire (MeSH), Australie (MeSH), Basidiomycota (classification), Basidiomycota (génétique), Biologie informatique (méthodes), Gènes fongiques (MeSH), Génome fongique (MeSH), Génomique (méthodes), Hordeum (microbiologie), Maladies des plantes (microbiologie), Phylogenèse (MeSH), Polymorphisme génétique (MeSH), Séquençage nucléotidique à haut débit (MeSH), Virulence (génétique).
- MESH :
- génétique : Basidiomycota, Virulence.
- microbiologie : Hordeum, Maladies des plantes.
- méthodes : Biologie informatique, Génomique.
- Analyse de séquence d'ADN, Annotation de séquence moléculaire, Australie, Basidiomycota, Gènes fongiques, Génome fongique, Phylogenèse, Polymorphisme génétique, Séquençage nucléotidique à haut débit.
- Wicri :
- geographic : Australie.
English descriptors
- KwdEn :
- Australia (MeSH), Basidiomycota (classification), Basidiomycota (genetics), Computational Biology (methods), Genes, Fungal (MeSH), Genome, Fungal (MeSH), Genomics (methods), High-Throughput Nucleotide Sequencing (MeSH), Hordeum (microbiology), Molecular Sequence Annotation (MeSH), Phylogeny (MeSH), Plant Diseases (microbiology), Polymorphism, Genetic (MeSH), Sequence Analysis, DNA (MeSH), Virulence (genetics).
- MESH :
- geographic : Australia.
- classification : Basidiomycota.
- genetics : Basidiomycota, Virulence.
- methods : Computational Biology, Genomics.
- microbiology : Hordeum, Plant Diseases.
- Genes, Fungal, Genome, Fungal, High-Throughput Nucleotide Sequencing, Molecular Sequence Annotation, Phylogeny, Polymorphism, Genetic, Sequence Analysis, DNA.
Abstract
Puccinia hordei (Ph) is a damaging pathogen of barley throughout the world. Despite its importance, almost nothing is known about the genomics of this pathogen, and a reference genome is lacking. In this study, the first reference genome was assembled for an Australian isolate of Ph ("Ph560") using long-read SMRT sequencing. A total of 838 contigs were assembled, with a total size of 207 Mbp. This included both haplotype collapsed and separated regions, consistent with an estimated haploid genome size of about 150Mbp. An annotation pipeline that combined RNA-Seq of Ph-infected host tissues and homology to proteins from four other Puccinia species predicted 25,543 gene models of which 1,450 genes were classified as encoding secreted proteins based on the prediction of a signal peptide and no transmembrane domain. Genome resequencing using short-read technology was conducted for four additional Australian strains, Ph612, Ph626, Ph608 and Ph584, which are considered to be simple mutational derivatives of Ph560 with added virulence to one or two of three barley leaf rust resistance genes (viz. Rph3, Rph13 and Rph19). To identify candidate genes for the corresponding avirulence genes AvrRph3, AvrRph13 and AvrRph19, genetic variation in predicted secreted protein genes between the strains was correlated to the virulence profiles of each, identifying 35, 29 and 46 candidates for AvrRph13, AvrRph3 and AvrRph19, respectively. The identification of these candidate genes provides a strong foundation for future efforts to isolate these three avirulence genes, investigate their biological properties, and develop new diagnostic tests for monitoring pathogen virulence.
DOI: 10.1534/g3.119.400450
PubMed: 31444296
PubMed Central: PMC6778787
Affiliations:
- Australie, République populaire de Chine
- Guangdong, Nouvelle-Galles du Sud
- Shenzhen, Sydney
- Université de Sydney
Links toward previous steps (curation, corpus...)
Le document en format XML
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Park</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia robert.park@sydney.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31444296</idno><idno type="pmid">31444296</idno><idno type="doi">10.1534/g3.119.400450</idno><idno type="pmc">PMC6778787</idno><idno type="wicri:Area/Main/Corpus">000152</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000152</idno><idno type="wicri:Area/Main/Curation">000152</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000152</idno><idno type="wicri:Area/Main/Exploration">000152</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en"><i>De Novo</i> Genome Assembly and Comparative Genomics of the Barley Leaf Rust Pathogen <i>Puccinia hordei</i> Identifies Candidates for Three Avirulence Genes.</title><author><name sortKey="Chen, Jiapeng" sort="Chen, Jiapeng" uniqKey="Chen J" first="Jiapeng" last="Chen">Jiapeng Chen</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Wu, Jingqin" sort="Wu, Jingqin" uniqKey="Wu J" first="Jingqin" last="Wu">Jingqin Wu</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Zhang, Peng" sort="Zhang, Peng" uniqKey="Zhang P" first="Peng" last="Zhang">Peng Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Dong, Chongmei" sort="Dong, Chongmei" uniqKey="Dong C" first="Chongmei" last="Dong">Chongmei Dong</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Upadhyaya, Narayana M" sort="Upadhyaya, Narayana M" uniqKey="Upadhyaya N" first="Narayana M" last="Upadhyaya">Narayana M. Upadhyaya</name><affiliation><nlm:affiliation>Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT, Australia, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Zhou, Qian" sort="Zhou, Qian" uniqKey="Zhou Q" first="Qian" last="Zhou">Qian Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Agricultural Genomic Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Agricultural Genomic Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124</wicri:regionArea><placeName><settlement type="city">Shenzhen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Dodds, Peter" sort="Dodds, Peter" uniqKey="Dodds P" first="Peter" last="Dodds">Peter Dodds</name><affiliation><nlm:affiliation>Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT, Australia, and.</nlm:affiliation><wicri:noCountry code="subField">and</wicri:noCountry></affiliation></author><author><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia robert.park@sydney.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></analytic><series><title level="j">G3 (Bethesda, Md.)</title><idno type="eISSN">2160-1836</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Australia (MeSH)</term><term>Basidiomycota (classification)</term><term>Basidiomycota (genetics)</term><term>Computational Biology (methods)</term><term>Genes, Fungal (MeSH)</term><term>Genome, Fungal (MeSH)</term><term>Genomics (methods)</term><term>High-Throughput Nucleotide Sequencing (MeSH)</term><term>Hordeum (microbiology)</term><term>Molecular Sequence Annotation (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Polymorphism, Genetic (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Virulence (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Annotation de séquence moléculaire (MeSH)</term><term>Australie (MeSH)</term><term>Basidiomycota (classification)</term><term>Basidiomycota (génétique)</term><term>Biologie informatique (méthodes)</term><term>Gènes fongiques (MeSH)</term><term>Génome fongique (MeSH)</term><term>Génomique (méthodes)</term><term>Hordeum (microbiologie)</term><term>Maladies des plantes (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Polymorphisme génétique (MeSH)</term><term>Séquençage nucléotidique à haut débit (MeSH)</term><term>Virulence (génétique)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Australia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term><term>Genomics</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Hordeum</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Hordeum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biologie informatique</term><term>Génomique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Fungal</term><term>Genome, Fungal</term><term>High-Throughput Nucleotide Sequencing</term><term>Molecular Sequence Annotation</term><term>Phylogeny</term><term>Polymorphism, Genetic</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Annotation de séquence moléculaire</term><term>Australie</term><term>Basidiomycota</term><term>Gènes fongiques</term><term>Génome fongique</term><term>Phylogenèse</term><term>Polymorphisme génétique</term><term>Séquençage nucléotidique à haut débit</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Australie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Puccinia hordei</i> (<i>Ph</i>) is a damaging pathogen of barley throughout the world. Despite its importance, almost nothing is known about the genomics of this pathogen, and a reference genome is lacking. In this study, the first reference genome was assembled for an Australian isolate of <i>Ph</i> ("<i>Ph</i>560") using long-read SMRT sequencing. A total of 838 contigs were assembled, with a total size of 207 Mbp. This included both haplotype collapsed and separated regions, consistent with an estimated haploid genome size of about 150Mbp. An annotation pipeline that combined RNA-Seq of <i>Ph</i>-infected host tissues and homology to proteins from four other <i>Puccinia</i> species predicted 25,543 gene models of which 1,450 genes were classified as encoding secreted proteins based on the prediction of a signal peptide and no transmembrane domain. Genome resequencing using short-read technology was conducted for four additional Australian strains, <i>Ph</i>612, <i>Ph</i>626, <i>Ph</i>608 and <i>Ph</i>584, which are considered to be simple mutational derivatives of <i>Ph</i>560 with added virulence to one or two of three barley leaf rust resistance genes (<i>viz. Rph3</i>, <i>Rph13</i> and <i>Rph19</i>). To identify candidate genes for the corresponding avirulence genes <i>AvrRph3</i>, <i>AvrRph13</i> and <i>AvrRph19</i>, genetic variation in predicted secreted protein genes between the strains was correlated to the virulence profiles of each, identifying 35, 29 and 46 candidates for <i>AvrRph13</i>, <i>AvrRph3</i> and <i>AvrRph19</i>, respectively. The identification of these candidate genes provides a strong foundation for future efforts to isolate these three avirulence genes, investigate their biological properties, and develop new diagnostic tests for monitoring pathogen virulence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31444296</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>12</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2160-1836</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>10</Month><Day>07</Day></PubDate></JournalIssue><Title>G3 (Bethesda, Md.)</Title><ISOAbbreviation>G3 (Bethesda)</ISOAbbreviation></Journal><ArticleTitle><i>De Novo</i> Genome Assembly and Comparative Genomics of the Barley Leaf Rust Pathogen <i>Puccinia hordei</i> Identifies Candidates for Three Avirulence Genes.</ArticleTitle><Pagination><MedlinePgn>3263-3271</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1534/g3.119.400450</ELocationID><Abstract><AbstractText><i>Puccinia hordei</i> (<i>Ph</i>) is a damaging pathogen of barley throughout the world. Despite its importance, almost nothing is known about the genomics of this pathogen, and a reference genome is lacking. In this study, the first reference genome was assembled for an Australian isolate of <i>Ph</i> ("<i>Ph</i>560") using long-read SMRT sequencing. A total of 838 contigs were assembled, with a total size of 207 Mbp. This included both haplotype collapsed and separated regions, consistent with an estimated haploid genome size of about 150Mbp. An annotation pipeline that combined RNA-Seq of <i>Ph</i>-infected host tissues and homology to proteins from four other <i>Puccinia</i> species predicted 25,543 gene models of which 1,450 genes were classified as encoding secreted proteins based on the prediction of a signal peptide and no transmembrane domain. Genome resequencing using short-read technology was conducted for four additional Australian strains, <i>Ph</i>612, <i>Ph</i>626, <i>Ph</i>608 and <i>Ph</i>584, which are considered to be simple mutational derivatives of <i>Ph</i>560 with added virulence to one or two of three barley leaf rust resistance genes (<i>viz. Rph3</i>, <i>Rph13</i> and <i>Rph19</i>). To identify candidate genes for the corresponding avirulence genes <i>AvrRph3</i>, <i>AvrRph13</i> and <i>AvrRph19</i>, genetic variation in predicted secreted protein genes between the strains was correlated to the virulence profiles of each, identifying 35, 29 and 46 candidates for <i>AvrRph13</i>, <i>AvrRph3</i> and <i>AvrRph19</i>, respectively. The identification of these candidate genes provides a strong foundation for future efforts to isolate these three avirulence genes, investigate their biological properties, and develop new diagnostic tests for monitoring pathogen virulence.</AbstractText><CopyrightInformation>Copyright © 2019 Chen et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jiapeng</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-9145-3018</Identifier><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jingqin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Peng</ForeName><Initials>P</Initials><Identifier Source="ORCID">0000-0002-4191-1068</Identifier><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Chongmei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Upadhyaya</LastName><ForeName>Narayana M</ForeName><Initials>NM</Initials><Identifier Source="ORCID">0000-0002-3052-0416</Identifier><AffiliationInfo><Affiliation>Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT, Australia, and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Agricultural Genomic Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dodds</LastName><ForeName>Peter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, Canberra, ACT, Australia, and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Robert F</ForeName><Initials>RF</Initials><Identifier Source="ORCID">0000-0002-9145-5371</Identifier><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Cobbitty, NSW, Australia robert.park@sydney.edu.au.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>G3 (Bethesda)</MedlineTA><NlmUniqueID>101566598</NlmUniqueID><ISSNLinking>2160-1836</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001315" MajorTopicYN="N" Type="Geographic">Australia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="Y">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016681" MajorTopicYN="Y">Genome, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D023281" MajorTopicYN="Y">Genomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059014" MajorTopicYN="N">High-Throughput Nucleotide Sequencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="N">Hordeum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence 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Chine</li></country><region><li>Guangdong</li><li>Nouvelle-Galles du Sud</li></region><settlement><li>Shenzhen</li><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><noCountry><name sortKey="Dodds, Peter" sort="Dodds, Peter" uniqKey="Dodds P" first="Peter" last="Dodds">Peter Dodds</name><name sortKey="Upadhyaya, Narayana M" sort="Upadhyaya, Narayana M" uniqKey="Upadhyaya N" first="Narayana M" last="Upadhyaya">Narayana M. Upadhyaya</name></noCountry><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Chen, Jiapeng" sort="Chen, Jiapeng" uniqKey="Chen J" first="Jiapeng" last="Chen">Jiapeng Chen</name></region><name sortKey="Dong, Chongmei" sort="Dong, Chongmei" uniqKey="Dong C" first="Chongmei" last="Dong">Chongmei Dong</name><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name><name sortKey="Wu, Jingqin" sort="Wu, Jingqin" uniqKey="Wu J" first="Jingqin" last="Wu">Jingqin Wu</name><name sortKey="Zhang, Peng" sort="Zhang, Peng" uniqKey="Zhang P" first="Peng" last="Zhang">Peng Zhang</name></country><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhou, Qian" sort="Zhou, Qian" uniqKey="Zhou Q" first="Qian" last="Zhou">Qian Zhou</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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